PACOCF3 (Palmityltrifluoromet?hylketone) is a selective phospholipase A2 inhibitor with an IC₅₀ of 3.8 μM. PACOCF3 alters Ca signaling in renal tubular cells.

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At a concentration of 20 μM, PACOCF3 does not change basal cytosolic free calcium concentrations ([Ca]_i), but at concentrations of 50-250 μM PACOCF3 induced an increase in [Ca]_i by activating extracellular Ca entry which is partly suppressed by 50 μM La3+.
The effect of PACOCF3 is abolished by removal of extracellular Ca. PACOCF3 (10 μM) enhances both the peak value and the area under the curve of the [Ca]_i increase induced by 10 μM ATP and 1 μM bradykinin by potentiating extracellular Ca influx without affecting internal Ca release.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

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• [1]. E J Ackermann, et al. Inhibition of macrophage Ca(2+)-independent phospholipase A2 by bromoenol lactone and trifluoromethyl ketones. J Biol Chem. 1995 Jan 6;270(1):445-50.  [Information]

  [2]. C R Jan, et al. Dual action of palmitoyl trifluoromethyl ketone (PACOCF3) on Ca2+ signaling: activation of extracellular Ca2+ influx and alteration of ATP- and bradykinin-induced Ca2+ responses in Madin Darby canine kidney cells. Arch Toxicol. 2000 Oct;74  [Information]

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