Pinostilbene (0- 40 μM; 24 hours, 48 hours) does not cause significant inhibition on the growth of normal colon cells.
Pinostilbene (20 μM, 40 μM; 24 hours, 48 hours) causes a significant and dose-dependent increase in the percentage of cells in S phase in both HCT116 and HT29 cells compared to the control cells.
Pinostilbene at μM also induces a modest increase of cell population in G2/M phase in HT29 cells.
Pinostilbene(20 μM, 40 μM; 24 hours, 48 hours) modulates expression of key signaling proteins related to cell proliferation and apoptosis.
Pinostilbene also acts as a resveratrol methylated derivative and displays protective effects against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay
Cell Line: |
HCT116 cells, HT29 cells |
Concentration: |
24 hours, 48 hours |
Incubation Time: |
0-100 μM |
Result: |
Inhibited the growth of two human colon cancer cells. |
Apoptosis Analysis
Cell Line: |
HCT116 cells, HT29 cells |
Concentration: |
20 μM, 40 μM |
Incubation Time: |
24 hours, 48 hours |
Result: |
Caused a significant and dose-dependent increase in the percentage of cells in S phase. |
Cell Viability Assay
Cell Line: |
HCT116 cells |
Concentration: |
20 μM,40 μM |
Incubation Time: |
24 hours, 48 hours |
Result: |
Significantly increased the expression levels of p53, Bax, cleaved caspase-3, cleaved PARP and p21, while decreased the expression levels of cyclin E and p-Rb. |