Corticosterone.|50-22-6|(皮质酮; 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)-陌孚医药/Medlife

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Corticosterone. (Synonyms: 皮质酮; 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)

目录号: PC11343 纯度: ≥98%

CAS No. :50-22-6

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中文名称

Corticosterone.

中文别名

肾上腺酮;皮质酮;皮质甾酮;肾上腺酮皮质甾酮;(11beta)-11,21-二羟基-4-孕烯-3,20-二酮;6-甲基-1-茚酮;Corticosterone 皮质甾酮;皮质酮(肾上腺酮) 标准品;皮质酮-[9,11,12,12-D4];皮质酮-[D4]氘代同位素内标;皮质脂酮;11β,21-二羟基-孕烯-3,20-酮;皮质甾酮分析标准品;11β,21-二羟基孕酮;可的松

英文名称

Corticosterone

英文别名

CORTICOSTERONE;NSC-9705;compoundb;Corticosteron;KENDALL'S 'B';Reichstein's B;17-Deoxycortisol;CORTICOSTERONE(RG);KENDALL'S COMPOUND B;(8S,9S,10R,11S,13S,14S,17S)-11-hydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one;Corticosterone solution;11β,21-Dihydroxyprogesterone;11beta,21-Dihydroxyprogesterone;4-Pregnene-11beta,21-diol-3,20-dione;NSC 9705;Reichstein's substance H;Compound B;11-Hydroxycorticoaldosterone;11,21-Dihydroxyprogesterone;11,12-Dihydroxyprogesterone;NSC9705;11-be

Cas No.

50-22-6

分子式

C₂₁H₃₀O₄

分子量

346.46

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

生物活性

Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect.

性状

Solid

IC50 & Target[1][2]

Human Endogenous Metabolite

体外研究(In Vitro)

Corticosterone (100 nM; 30 min) via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane.
Corticosterone (CORT) (1 μM; 48 h) shows good immunosuppressive properties (functionally compromises maturation of BMDC), which impairs LPS-induced up-regulation of maturation-associated markers (MHC class II, B7.2, B7.1 and CD40).

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	HEK293 cells
Concentration:	100 nM
Incubation Time:	30 min
Result:	Caused a significant enhancement of mEPSC amplitude (mEPSC represents the postsynaptic response to release of individual vesicles of glutamate). Increased the transmission of glutamatergic, and increased synaptic AMPAR currents via a Rab4-dependent mechanism. Profoundly increased surface GluR1 cluster density, cluster size and cluster fluorescence intensity. Significantly increased the amount of Rab4 that binded to WT-GDI, S45A-GDI, or S121A-GDI but not S213A-GDI. Induced the phosphorylation of GST-GDI, GST-GDI, and GST-GDI, but not GST-GDI, and this effect was blocked in cells transfected with SGK1 small interfering RNA. Increased AMPAR surface expression via a mechanism dependent on GDI phosphorylation.

Cell Viability Assay

Cell Line:	BMDC cells
Concentration:	1 μM
Incubation Time:	48 h
Result:	Completely blocked the expression of MHC class II and B7.2 that induced by LPS, and maximally impaired BMDC cells maturation at 12 h. Reduced B7.1 by 50%, and slightly down-regulated CD40.

体内研究(In Vivo)

Corticosterone results in a marked reduction in the ability of BMDC cells to prime naive CD8 T cells in vivo.
Corticosterone (0.03 or 1 mg/kg; s.c.; single) downregulates expression of BDNF mRNA in dentate gyrus and CA1 of rats.
Corticosterone (2.6 mg/kg; in animal feedings; 8 days) restores ethanol intake and preference to approximately normal preoperative levels in adrenalectomy (ADX) rats.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male Wistar rats (150-170 g; adrenalectomized).
Dosage:	0.03 or 1 mg/kg
Administration:	Subcutaneous injection; single.
Result:	Decreased expression of BDNF mRNA in dentate gyrus, with 25% and 50% lower for dosages of 0.03 and 1 mg/kg, respectively (3 h after administration). Reduced approximately 40% BDNF mRNA level as compared to the t=0 h control group (3 h after administration), but the level increased by 100% when 12 h after administration (compared to t=3 h and t=6 h group).

Animal Model:	Male Wistar rats (3-week-old; adrenalectomized).
Dosage:	2.6 mg/kg
Administration:	In animal feedings; 8 days.
Result:	Restored ethanol intake and preference of adrenalectomy (ADX) rats to approximately normal preoperative levels and to the levels observed in the sham-operated group (SH) rats.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

ClinicalTrial

结构分类

Steroids

参考文献

[1]. Elftman MD, et al. Corticosterone impairs dendritic cell maturation and function. Immunology. 2007 Oct;122(2):279-90. [Information]

[2]. Schaaf MJ, et al. Downregulation of BDNF mRNA and protein in the rat hippocampus by corticosterone. Brain Res. 1998 Nov 30;813(1):112-20. [Information]

[3]. Fahlke C, et al. Involvement of corticosterone in the modulation of ethanol consumption in the rat. Alcohol. 1994 May-Jun;11(3):195-202. [Information]

溶解度数据

体外研究:

DMSO : 100 mg/mL (288.63 mM; Need ultrasonic)

Ethanol : 14.29 mg/mL (41.25 mM; Need ultrasonic)

H₂O : 0.67 mg/mL (1.93 mM; Need ultrasonic)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8863 mL	14.4317 mL	28.8634 mL
5 mM	0.5773 mL	2.8863 mL	5.7727 mL
10 mM	0.2886 mL	1.4432 mL	2.8863 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： 5% Cremophor EL 95% (20% HP-β-CD)
Solubility: 5 mg/mL (14.43 mM); Clear solution; Need ultrasonic
2.

建议依照次序添加每种溶剂： 20% HP-β-CD in saline
Solubility: 4 mg/mL (11.55 mM); Suspended solution; Need ultrasonic
3.

建议依照次序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.22 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液
4.

建议依照次序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.22 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
5.

建议依照次序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.22 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
6.

建议依照次序添加每种溶剂： 0.5% CMC-Na/saline water
Solubility: 2 mg/mL (5.77 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C
7.

建议依照次序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

此方案可获得 ≥ 1.43 mg/mL (4.13 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液
8.

建议依照次序添加每种溶剂： 10% EtOH 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

此方案可获得 ≥ 1.43 mg/mL (4.13 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 EtOH 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
9.

建议依照次序添加每种溶剂： 10% EtOH 90% corn oil
Solubility: ≥ 1.43 mg/mL (4.13 mM); Clear solution

此方案可获得 ≥ 1.43 mg/mL (4.13 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中，混合均匀。

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Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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