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硝呋酚酰肼 (Synonyms: 硝呋齐特)
目录号: PC16544 纯度: ≥98%
CAS No. :965-52-6
商品编号 规格 价格 会员价 是否有货 数量
PC16544-200mg 200mg ¥509.60 请登录
PC16544-500mg 500mg ¥744.80 请登录
PC16544-10mM (in 1mL DMSO) 10mM (in 1mL DMSO) ¥539.00 请登录
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中文名称
硝呋酚酰肼
中文别名
硝呋酚酰肼;哨呋齐特杂质;硝呋酚酰阱;硝呋酚酰肼 标准品;硝呋酚酰肼(硝呋齐特);硝呋酚酰肼杂质;硝呋齐特;硝呋齐特 EP标准品;硝呋齐特 标准品;4-羟基-2'-(5-硝基呋喃甲叉)-苯酰肼;硝呋奇特
英文名称
Nifuroxazide
英文别名
nifuroxazide;4-hydroxy-N-[(E)-(5-nitrofuran-2-yl)methylideneamino]benzamide;Nifuroxazide-D4;Diarlidan;Dicoferin;Ercefurol;Ercefuryl;Nifuroxazid;Nifuroxazida;Nifuroxazidum;Nifuroxazidum [INN-Latin];p-hydroxy-N'-(5-nitrofurfurylidene)benzhydrazide;p-Hydroxybenzoic acid (5-nitrofurfurylidene) hydrazide;Nifruoxazide;Nifuroxazida [INN-Spanish];RC 27109;p-Hydroxybenzoic acid (5-nitrofurfurylidene)hydrazide;PM5LI0P38J;MLS000069620;C12H9N3O5;4-Hydroxy-N'-(5-nitrofurfuryliden)benzhydrazid;SMR000058185;4-hydroxy-N-[(E)-(5-nitro-2-furyl)methyleneamino]benzamide;4-hydroxy-N-[(E)-(5-nitrofuran-2-yl)m
Cas No.
965-52-6
分子式
C12N3O5H9
分子量
275.22
包装储存
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
生物活性

Nifuroxazide is an effective inhibitor of STAT3, also exerts potent anti-tumor and anti-metastasis activity.

性状

Solid

IC50 & Target[1][2]

STAT3

 

体外研究(In Vitro)

When U266 cells are incubated with Nifuroxazide, a significant dose-dependent decrease in STAT3 tyrosine phosphorylation is observed. This inhibition of STAT3 tyrosine phosphorylation is rapid, occurring as early as 1 h after treatment, and is sustained for at least 24 h. Treatment of U266 or INA6 cells with Nifuroxazide for 48 hours result in a dose-dependent loss of cell viability with an EC50 of approximately 4.5 μM in both cell types. Notably, the MM cells lacking constitutive STAT3 activation show little toxicity to Nifuroxazide.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究(In Vivo)

Compared with the vehicle group, treatment with Nifuroxazide could inhibit tumor growth and tumor weight in a dose-dependent manner, with the inhibition rate of tumor volumes being 43.0% and 62.1% at 25 mg/kg and 50 mg/kg, respectively. It is also shown that Nifuroxazide significantly inhibits the proliferation of nuclear Ki-67-positive cells and induces apoptosis cells of cleaved caspase-3-positive cells. Besides, it is found that treatment with Nifuroxazide could inhibit the expression of MMP-2, MMP-9 and p-Stat3 in A375 tumor tissues. What’s more, Nifuroxazide inhibits the infiltration of MDSCs into the lung, which might be associated with suppression of distant colonization of tumor cells in B16-F10 melanoma metastasis model.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

运输条件

Room temperature or refrigerated transportation.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
参考文献
溶解度数据
体外研究: 

DMSO : ≥ 155 mg/mL (563.19 mM)

* "≥" means soluble, but saturation unknown.

配制储备溶液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.6335 mL 18.1673 mL 36.3346 mL
5 mM 0.7267 mL 3.6335 mL 7.2669 mL
10 mM 0.3633 mL 1.8167 mL 3.6335 mL
*

产品不同,其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液;配成溶液后,建议分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,建议在 6 个月内使用,-20°C 储存时,建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    建议依照次序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (9.37 mM); Suspended solution

    此方案可获得 ≥ 2.58 mg/mL (9.37 mM,饱和度未知) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH?O 中,得到澄清透明的生理盐水溶液
  • 2.

    建议依照次序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.58 mg/mL (9.37 mM); Suspended solution

    此方案可获得 ≥ 2.58 mg/mL (9.37 mM,饱和度未知) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*
搜索质检报告(COA)

1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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