BCECF-AM|117464-70-7|-陌孚医药/Medlife

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BCECF-AM

目录号: PC15060 纯度: ≥98%

CAS No. :117464-70-7

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中文名称	BCECF-AM
中文别名	2',7'-二(2-羧基乙基)-5(6)-羧基荧光素乙酰氧甲基酯;2'',7''-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯;2,7-二(2-羧基乙基)-5(6)-羧基荧光素乙酸甲酯;2'',7''-二(2-羧基乙基)-5(6)-羧基荧光素乙酰氧甲基酯;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯;2,7-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯,Mixed isomers;2`,7`-双(2-羧基乙基)-5(6)-羧基荧光素四(乙酰氧甲基)酯;顺,顺-已二烯二酸;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯,1 mg;2',7'-双(2-羧基乙基)-5(6)-羧基荧光素四(乙酰氧甲基)酯;PH荧光探针BCECF, AM;BCECF--1M 1MG
英文名称	BCECF-AM
英文别名	Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid, 3',6'-bis(acetyloxy)-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-,2',7'-bis[(acetyloxy)methyl] ester;2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet;2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet;BCECF (AM);BCECF, AM ester;BCECF-Acetoxymethyl ester;BCECF-AM;Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid, 3',6'-bis(acetyloxy)-5(or 6)...;2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester;2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester Mixed isomers;acetyloxymethyl 3',6'-bis(acetyloxymethoxy)-2',7'-bis[3-(acetyloxymethoxy)-3-oxopropyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate;BCECF-acetoxymethyl;mL in DMSO;2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester,1 mg;BCECF--1M;Bcecf-AmSolution;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescei;2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein AM ester;2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester;BCECF.AM ESTER, 1 MG;ML INANHYDROUS DMSO;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO
Cas No.	117464-70-7
分子式	C₄₂O₂₁H₄₀
分子量	880.75
包装储存	-20°C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。

生物活性	BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH.
性状	Solid
体外研究(In Vitro)	Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K/H ionophor nigericin does not inhibit hydrogenosomal loading with BCECF-AM. The pH-sensitive fluorescent dyes to measure cytosolic pH. 1.Prepare a 2 to 20 mM stock solution of BCECF-AM in DMSO. 2.Prepare a 5-50 μM BCECF-AM dye-loading solution in buffer solutions (HHBS or PBS). 3. Add 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate) BCECF-AM dye-loading solution into the cell plate. 4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes. 5. Wash and replace the dye-loading solution with buffers. 6. Run the pH assay by monitoring the fluorescence at E_x/E_m = 490/535 nm or 430/535 nm for ratio measurements. Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件	Room temperature or refrigerated transportation.
储存方式	-20°C, protect from light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
Emission(Em)	Em 495-570 nm Green
Excitation(Ex)	Ex 450-495 nm Blue
参考文献	[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2,7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45. [Information] [2]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303.

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1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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