3-Methyladenine.|5142-23-4|(3-甲基腺嘌呤; 3-MA)-陌孚医药/Medlife

您当前的位置：

3-Methyladenine. (Synonyms: 3-甲基腺嘌呤; 3-MA)

目录号: PC16216 纯度: ≥99%

CAS No. :5142-23-4

商品编号	规格	价格	会员价
PC16216-50mg	50mg	¥445.00	请登录
PC16216-100mg	100mg	¥665.00	请登录
PC16216-200mg	200mg	¥1150.00	请登录
PC16216-500mg	500mg	¥2320.00	请登录
PC16216-10mM (in 1mL DMSO)	10mM (in 1mL DMSO)	¥135.00	请登录

Medlife所售产品仅用于科学研究（非临床研究），非药品不可食用，不可用于人体或动物的临床诊断和治疗，我们不为个人提供产品及服务。产品COA等资料，可至下方“质量控制”中下载。

加入购物车在线咨询收藏产品大包装询价

中文名称

3-Methyladenine.

中文别名

6-氨基-3-甲基嘌呤;3-甲基腺嘌呤;3-Methyladenine 3-甲基腺嘌呤;3-甲基腺素;6-氨基-3-甲基嘌呤、3-甲基腺嘌呤;NSC66389 抑制剂;邻二氮杂菲;1,10-菲罗啉(无水);2,9-二甲基啉菲罗啉,新铜试剂;3-甲基-3H-嘌呤-6-胺;6-氨基-3-甲基嘌呤(3MA);细胞自噬抑制剂

英文名称

3-Methyladenine

英文别名

3-METHYLADENINE;3H-Purin-6-amine,3-methyl-;3-MA;3-Methyl-3H-purin-6-amine;3-Methyladenine (3-MA);3-methylpurin-6-amine;6-?Amino-?3-?methylpurine;3-MA,6-Amino-3-methylpurine;6-Amino-3-methylpurine;3-Methyl-6-aminopurine;N3-Methyladenine;NSC 66389;3H-Purin-6-amine, 3-methyl-;3-Methyl-3H-adenine;ADENINE, 3-METHYL-;3-METHYL-3H-PURIN-6-YLAMINE;3-Methyladenine autophagy inhibitor;3mea;4aia;3-methyl-Adenine;NSC66389;1p7m;4ai5;Autophagy Inhibitor, 3-MA;NCIOpen2_000270;SCHEMB

Cas No.

5142-23-4

分子式

C₆H₇N₅

分子量

149.15

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

生物活性

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

性状

Solid

IC50 & Target[1][2]

PtdIns3Kγ

60 μM (IC₅₀, Cell Assay)

Vps34

25 μM (IC₅₀, Cell Assay)

Autophagy

Mitophagy

Human Endogenous Metabolite

体外研究(In Vitro)

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner.
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions.
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy.
3-Methyladenine induces cell death is independent of autophagy inhibition.
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest.
Note:
在体外细胞培养实验中，3-MA 常用的浓度为 0.5-10 mM，所以优先推荐直接称取本次实验所需粉末量，用培养基完全溶解，0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用，尽快用完。

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	HeLa cells
Concentration:	0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time:	0 hour, 24 hours and 48 hours
Result:	Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	24 hours
Result:	Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	0 hour, 12 hours, 24 hours and 48 hours
Result:	Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.

体内研究(In Vivo)

3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours.
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP.
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)
Dosage:	1.5?mg/100?g (1000 μM)
Administration:	Intraperitoneal injection
Result:	Alleviated Sodium Taurocholate-Induced SAP.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

结构分类

Others

参考文献

[1]. Miller S, et al. Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy. 2010 Aug;6(6):805-7. [Information]

[2]. Hou H, et al. Inhibitors of phosphatidylinositol 3-kinases promote mitotic cell death in HeLa cells. PLoS One. 2012;7(4):e35665. [Information]

[3]. Wang X, et al. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats.Mediators Inflamm. 2016;2016:8369704. [Information]

溶解度数据

体外研究:

H₂O : 5 mg/mL (33.52 mM; Need ultrasonic)

DMSO : 8.33 mg/mL (55.85 mM; Need ultrasonic)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	6.7047 mL	33.5233 mL	67.0466 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： PBS
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic

搜索质检报告(COA)

产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

相关产品

The molarity calculator equation

The dilution calculator equation