Motilin (26-47), human, porcine is an endogenous motilin receptor ligand with K_i and EC₅₀ of 2.3 nM and 0.3 nM in a Chinese hamster ovary cell line.

Solid

EC50: 0.3 nM (motilin receptor, Chinese hamster ovary cell)
Ki: 2.3 nM (motilin receptor, Chinese hamster ovary cell)

[Bpa,Ile]motilin is a full agonist at the motilin receptor that increases intracellular calcium in a concentration-dependent manner (EC₅₀ = 1.5 ± 0.4 nM). Motilin binds with high affinity (IC₅₀ 0.7± 0.2 nM) to receptors on smooth muscle cells. Motilin selectively activates G(q) and G(13), stimulates G alpha(q)-dependent phosphoinositide (PI) hydrolysis and 1,4,5-trisphosphate (IP(3))-dependent Ca release, and increases cytosolic free Ca. Motilin induces a biphasic, concentration-dependent contraction (EC₅₀ = 1.0 +/- 0.2 nM), consisting of an initial peak followed by a sustained contraction.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature or refrigerated transportation.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

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Phe-Val-Pro-Ile-Phe-Thr-Tyr-Gly-Glu-Leu-Gln-Arg-Met-Gln-Glu-Lys-Glu-Arg-Asn-Lys-Gly-Gln

• [1]. Coulie B, et al. Identification of peptide ligand-binding domains within the human motilin receptor using photoaffinity labeling. J Biol Chem. 2001 Sep 21;276(38):35518-22. Epub 2001 Jul 18.  [Information]

  [2]. Huang J, et al. Signaling pathways mediating gastrointestinal smooth muscle contraction and MLC20 phosphorylation by motilin receptors. Am J Physiol Gastrointest Liver Physiol. 2005 Jan;288(1):G23-31.  [Information]