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Norgestrel (Synonyms: 炔诺孕酮)
目录号: PC18045 纯度: ≥98%
CAS No. :6533-00-2
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中文名称
Norgestrel
中文别名
炔诺孕酮;18-甲基炔诺酮;DL-甲基炔诺酮;高诺酮;药典标准品;左炔孕酮;甲基炔诺酮,18-甲炔诺酮;甲基炔诺酮;炔诺酮;炔诺酮-D6;炔诺孕酮 USP标准品;炔诺孕酮标准品(JP)
英文名称
Norgestrel
英文别名
Norgestrel;Norgestrel [USAN:INN:BAN:JAN];(+-)-13-Ethyl-17-hydroxy-18,19-dinor-17alpha-pregn-4-en-20-yn-3-one;dl-13-beta-Ethyl-17-alpha-ethynyl-17-beta-hydroxygon-4-en-3-one;dl-13-beta-Ethyl-17-alpha-ethynyl-19-nortestosterone;18,19-Dinor-17-alpha-pregn-4-en-20-yn-3-one, 13-ethyl-17-hydroxy-, (+-)-;18,19-Dinorpregn-4-en-20-yn-3-one, 13-ethyl-17-hydroxy-,(17alpha)-(+-)-;Norgestrel (DL-);Levonorgestrel;(±)-Norgestrel;fh122-a;monovar;Neogest;NORPLANT;Ovrette;sh850;wy3707;(17α)-(±)-13-Ethyl-17-hydroxy-18,19-dinorpregn-4-en-20-yn-3-one;DL-13b-Ethyl-17a-ethynyl-17b-hydroxygon-4-en-3-one
Cas No.
6533-00-2
分子式
C21H28O2
分子量
312.45
包装储存
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
生物活性

Norgestrel is a synthetic analog of progesterone, a compound commonly found in oral contraceptive pill, and a powerful neuroprotective antioxidant, preventing light-induced ROS in photoreceptor cells, and cell death.

性状

Solid

体外研究(In Vitro)

Norgestrel (20 μM; 24 hours; 661W cells) treatment significantly increases cellular viability after serum deprivation and so it is demonstrated that Norgestrel is neuroprotective to stressed 661W cells.
Norgestrel (20 μM; 24 hours; 661W cells) treatment decreases apoptotic induced cleavage of PARP and caspase-3.
Norgestrel (20 μM; 6 hours; 661W cells) treatment bFGF is results in a significant upregulation of bFGF mRNA in photoreceptor cells.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line: 661W cells
Concentration: 20 μM
Incubation Time: 24 hours
Result: Significantly increased cellular viability after serum deprivation.

Western Blot Analysis

Cell Line: 661W cells
Concentration: 20 μM
Incubation Time: 24 hours
Result: Decreased apoptotic induced cleavage of PARP and caspase-3.

RT-PCR

Cell Line: 661W cells
Concentration: 20 μM
Incubation Time: 6 hours
Result: A significant upregulation of bFGF mRNA over 1 hour.
体内研究(In Vivo)

Norgestrel (100 mg/kg; intraperitoneal injection; for 6, 24 or 48 hours; Balb/c mice) treatment can prevent light-induced ROS in photoreceptor cells, and subsequent cell death. Norgestrel acts via post-translational modulation of the major antioxidant transcription factor Nrf2; bringing about its phosphorylation, subsequent nuclear translocation, and increases levels of its effector protein superoxide dismutase 2 (SOD2).

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice are born into and maintained in dim cyclic light
Dosage: 100 mg/kg;
Administration: Intraperitoneal injection; for 6, 24 or 48 hours
Result: Increased expression and activation of Nrf2 via phosphorylation on serine 40, increased expression of its target antioxidant superoxide dismutase 2 (SOD2), and reduced mitochondrial oxidative stress.
运输条件

Room temperature or refrigerated transportation.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
ClinicalTrial
参考文献
溶解度数据
体外研究: 

DMSO : 100 mg/mL (320.05 mM; Need ultrasonic)

配制储备溶液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2005 mL 16.0026 mL 32.0051 mL
5 mM 0.6401 mL 3.2005 mL 6.4010 mL
10 mM 0.3201 mL 1.6003 mL 3.2005 mL
*

产品不同,其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液;配成溶液后,建议分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,建议在 6 个月内使用,-20°C 储存时,建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    建议依照次序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH?O 中,得到澄清透明的生理盐水溶液
  • 2.

    建议依照次序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    建议依照次序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.00 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*
搜索质检报告(COA)

1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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