Aphidicolin (0.5 μM, 5 μM; 0-5 d) selectively kills neuroblastoma cells, but shows moderate cytotoxicity on normal human embryonal cells and HeLa, H9, A549 and Caco-2 cell lines.
Aphidicolin (0.4 μg/mL; 3 d) arrests cell cycle at G2 phase.
Aphidicolin (100 nM-10 μM; 48 h) inhibits cell proliferation via the p53-GADD45β pathway and (1 μM; 24 h) induces apoptosis in AtT-20 cells.
Aphidicolin (10 μM; 0-6 h) decreases the phosphorylation of Akt, (100 nM-10 μM; 24 h) increases the mRNA levels of the stress response gene growth arrest and DNA damage-inducible 45β (GADD45β), a putative downstream target of p53.
Aphidicolin (10 μM; 0-6 h) inhibits Varicella-zoster virus (VZV) with EC50s of 0.5-0.6 μM, with low cytotoxicity.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cytotoxicity Assay
Cell Line: |
NB cell lines: UKF-NB-1/2/3 and IMR-32 |
Concentration: |
0.5 μM, 5μM |
Incubation Time: |
1, 2, 3, 4, 5 days |
Result: |
Resulted in cellular enlargement and extension of cellular processes before cell lysis occurred.
|
Cell Cycle Analysis
Cell Line: |
Normal human diploid cells |
Concentration: |
0.4 μg/mL |
Incubation Time: |
3 days or 7 days |
Result: |
Resulted more than 80% of the cells moved through S phase and were accumulated at G2 phase.
Inbihited the growth of the cells completely without causing apparent cell death.
|
Western Blot Analysis
Cell Line: |
AtT-20 cells pituitary corticotroph tumor cells |
Concentration: |
10 μM |
Incubation Time: |
0 min, 5 min, 30 min, 2 h, 6 h, 24 h |
Result: |
Inhibited Akt phosphorylation in AtT-20 cells during 5 min-2 h, in a time-dependent manner.
Increased protein level of p27 during 30 min-6 h, and remarkably increased p53 level at 24 h. |