C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex (IC₅₀=0.88 μM).

Solid

0.88 μM (USP1)

Pretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC₅₀ of 0.88±0.03 μM. C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes 体外研究. However, the IC₅₀ of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin. C527 treatments lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature or refrigerated transportation.

Sealed and stored at 4℃

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Mistry H, et al. Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther. 2013 Dec;12(12):2651-62.  [Information]