Ziyuglycoside II is a triterpenoid saponin compound extracted from Sanguisorba officinalis L.. Ziyuglycoside II induces reactive oxygen species (ROS) production and apoptosis. Anti-inflammation and anti-cancer effect.
性状
Solid
体外研究(In Vitro)
Ziyuglycoside II (10-60 μM; 24 h and 48 h) inhibits MDA-MB-435 cells growth in a dose-dependent manner. The IC50 of Ziyuglycoside II at 24 h and 48 h is 5.92 μM and 4.74 μM, respectively.
Ziyuglycoside II (5-25 μM) induces G0/G1 and S phase arrest in MDA-MB-435 cells at 24 h.
Ziyuglycoside II (5-25 μM; 24 hours) significantly increases apoptotic rate of MDA-MB-435 cells.
Ziyuglycoside II (5-25 μM; 24 hours) increases expressions of both p53 and p21 in MDA-MB-435 cells, which effect is dose-dependent.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay
Cell Line:
MDA-MB-435 cells
Concentration:
10, 20, 30, 40, 50, 60 μM
Incubation Time:
24 hours and 48 hours
Result:
The IC50 at 24 h and 48 h was 5.92 μM and 4.74 μM, respectively.
Cell Cycle Analysis
Cell Line:
MDA-MB-435 cells
Concentration:
5, 10, 25 μM
Incubation Time:
24 hours
Result:
Induced G0/G1 and S phase arrest.
Apoptosis Analysis
Cell Line:
MDA-MB-435 cells
Concentration:
5, 10, 25 μM
Incubation Time:
24 hours
Result:
The apoptotic rate was significantly increased in comparison to that of the control.
Western Blot Analysis
Cell Line:
MDA-MB-435 cells
Concentration:
5, 10, 25 μM
Incubation Time:
24 hours
Result:
Treatment resulted in increased expressions of both p53 and p21.