Bafilomycin C1|88979-61-7|(巴菲霉素C1)-陌孚医药/Medlife

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Bafilomycin C1 (Synonyms: 巴菲霉素C1)

目录号: PC12431 纯度: ≥98%

CAS No. :88979-61-7

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中文名称	Bafilomycin C1
中文别名	巴佛罗霉素C1;巴佛洛霉素C 1;巴伐洛霉素 C1
英文名称	Bafilomycin C1
英文别名	2-Butenedioic acid(2E)-,1-[(2R,4R,5S,6R)-tetrahydro-2-hydroxy-2-[(1S,2R,3S)-2-hydroxy-3-[(2R,3S,4E,6E,9S,10S,11R,12E,14Z)-10-hydroxy-3,15-dimethoxy-7,9,11,13-tetramethyl-16-oxooxacyclohexadeca-4,6,12,14-tetraen-2-yl]-1-methylbutyl]-5-methyl-6-(1-methylethyl)-2H-pyran-4-yl]ester;BAFILOMYCIN C1;2-Demethyl-2-methoxy-24-methylhygrolidin;L 681110A1
Cas No.	88979-61-7
分子式	C₃₉H₆₀O₁₂
分子量	720.89
包装储存	-20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

生物活性

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC).

性状

Solid

IC50 & Target[1][2]

Macrolide

体外研究(In Vitro)

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner.
Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells.
Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	SMMC7721 cell and HepG2 cell
Concentration:	0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721 1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time:	6 days
Result:	Retarded the cell growth.

Western Blot Analysis

Cell Line:	SMMC7721 cells
Concentration:	3.3 μM
Incubation Time:	24 hours
Result:	Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis

Cell Line:	SMMC7721 and HepG2 cells
Concentration:	3.3 μM; 10 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in SMMC7721 and HepG2 cells.

体内研究(In Vivo)

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10 cells/100 μL)
Dosage:	0.2 mg/kg
Administration:	Subcutaneous injection; 20 days
Result:	Suppressed tumor growth of SMMC7721 tumor xenografts.

运输条件

Room temperature or refrigerated transportation.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

结构分类

来源

Streptomyces sp

参考文献

[1]. E J Bowman, et al. Bafilomycins: A Class of Inhibitors of Membrane ATPases From Microorganisms, Animal Cells, and Plant Cells. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7972-6. [Information]

[2]. Xiaoxiao Gao,et al.Bafilomycin C1 Induces G0/G1 Cell-Cycle Arrest and Mitochondrial-Mediated Apoptosis in Human Hepatocellular Cancer SMMC7721 Cells. J Antibiot (Tokyo). 2018 Sep;71(9):808-817. [Information]

溶解度数据	体内研究: 1. Bafilomycin C1 (0.2 mg/kg body weight, dissolved in 60% 1,2-propanediol)

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Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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