AZ 628 reduces activities of preactivated B-Raf, B-RafV600E, and c-Raf-1 in 体外研究 kinase assays, with IC50 values of 105, 34 and 29 nM, respectively. AZ 628 also inhibits activation of number of tyrosine protein kinases including VEGFR2, DDR2, Lyn, Flt1, FMS and others. AZ 628 inhibits anchorage-dependent and -independent growth, causes cell cycle arrest, and induces apoptosis in colon and melanoma cell lines harboring B-RafV600E mutation. AZ 628 suppresses growth in cells expressing K-RAS. Inhibition of RAF with AZ 628 suppresses MEK and ERK phosphorylation. AZ 628 selectively affects viability in K-RAS mutant cells.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.