4-Nitrophenyl α-D-galactopyranoside (Synonyms: PNP-alpha-D-Gal; PNP-α-D-Gal)
目录号: PL13994 纯度: ≥98%
CAS No. :7493-95-0
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中文名称
4-Nitrophenyl α-D-galactopyranoside
中文别名
4-硝基苯基 alpha-D-吡喃半乳糖苷;4-硝基苯-α-D-吡喃半乳糖苷;4-硝基苯基-α-D-吡喃半乳糖苷;对硝基苯基-α-D-吡喃半乳糖苷;4-硝基苯基α-D-吡喃半乳糖苷[α-D-牛乳糖用培养基];PNPG,对硝基苯-α-D-半乳糖苷;-硝基苯基α-D-吡喃半乳糖苷[α-D-牛乳糖用培养基];4-硝基苯-ALPHA-D-半乳糖苷;4-硝基苯-Α-D-半乳糖苷(PNPG);4-硝基苯基-ALPHA-D-半乳糖苷;4-硝基苯基-半乳糖苷;PNPG,4-硝基苯-α-D-吡喃半乳糖苷;PNPG,对硝基苯-Α-D-吡喃葡萄糖苷;PNPG糖苷;对硝基苯-ALPHA-D-半乳糖苷;对硝基苯-α-D-半乳糖苷
英文名称
4-Nitrophenyl α-D-galactopyranoside
英文别名
4-Nitrophenyl alpha-D-galactopyranoside;4-NITROPHENYL-ALPHA-D-GALACTOPYRANOSIDE;4-Nitrophenyl α-D-galacto-pyran-oside;4-Nitrophenyl alpha-D-Galactopyranoside [Substrate for alpha-D-Galactosidase];4-Nitrophenyl a-D-galactopyranoside;4-Nitrophenyl α-D-galactopyranoside;NITROPHENYL-a-D-GALACTOPYRANOSIDE, 4-(RG);p-Nitrophenyl .α.-D-galactopyranoside;p-Nitrophenyl α-D-Ga;p-Nitrophenyl α-D-Galactopyranoside;P-NITROPHENYL-ALPHA-D -GALACTOPYRANOSIDE CRYSTALLINE;4-Nitrophenyl α-D-galacto­pyran­oside;amp;GAL1-A-PNP;oside;PNPG;pNP-galactopyranoside;pyran­Galactopyranoside,p-nitrophenyl, a-D-(8CI);4-Nitrophenyl a-D-galactoside;NSC 89286;p-Nitrophenyl a-D-galactopyranoside;p-Nitrophenyl a-D-galactoside;p-Nitrophenyl a-galactopyranoside;p-Nitrophenyl a-galactoside;p-NITROPHENYL-alpha-D-GALACTOPYRANOSIDE;4-nitrophenyl ;A-d-galactopyranoside;p-Nitrophenyl alpha-D-galactopyranoside;p-nitrophenyl-alpha-D-galactoside;4-nitrophenyl-alpha-D-galactoside;1-O-(p-nitrophenyl)-alpha-D-galactose;1-O-(4-nitrophenyl)-alpha-D-galactose;1-O-(4-nitrophenyl)-alpha-D-galactopyranose;1-O-(p-nitrophenyl)-alpha-D-galactopyranose;PNP-alpha-D-Gal;PNP-α-D-Gal
Cas No.
7493-95-0
分子式
C12H15NO8
分子量
301.25
包装储存
Powder -20°C 3 years;4°C 2 years
产品详情
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) 是一种 4-硝基苯基 (pNP) 糖吡喃糖苷的人工底物,用于检测 α-半乳糖苷酶活性。当 4-Nitrophenyl α-D-galactopyranoside 用作底物时,释放的 pNP 量会显着增加。
生物活性
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when 4-Nitrophenyl α-D-galactopyranoside is used as substrates.
性状
Solid
体外研究(In Vitro)
Using 4-nitorophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 whereas no activity was detected at pH 7.0.
The digestion assays using 4-nitrophenyl (pNP) glycopyranosides as artificial substrates shows that yieldin liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years;4°C 2 years
参考文献
[1]. Okamoto-Nakazato A. Implications of the galactosidase activity of yieldin in the regulatory mechanism of yield threshold that is fundamental to cell wall extension. Physiol Plant. 2018 Jun;163(2):259-266.
溶解度数据
In Vitro: H2O : 16.67 mg/mL (55.34 mM; ultrasonic and warming and heat to 60°C)配制储备液
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1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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