Photo-lysine hydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

Solid

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and ch

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

[1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

In Vitro: H₂O : ≥ 40 mg/mL (163.19 mM)配制储备液