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产品总数：60953
| 中文名称 |
BODIPY 493/503
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|---|---|
| 中文别名 |
[[(3,5-二甲基-1H-吡咯-2-基)(3,5-二甲基-2H-吡咯-2-亚基)甲基]甲烷](二氟硼烷);4,4-二氟-1,3,5,7,8-五甲基-4-硼-3A,4A-二氮杂-S-茚烯;吡咯546*荧光参照标准*;盐酸他克林;吡咯甲川546;1,3,5,7,8-五甲基-4,4-二氟-4-硼杂-3a,4a-二氮杂-s-引达省;1,3,5,7,8-五甲基-4,4-二氟-4-硼杂-3A,4A-二氮杂-S-引达省;[[(3,5-二甲基-1H-吡咯-2-基)(3,5-二甲基-2H-吡咯-2-亚基)甲基]甲烷](二氟硼烷)(>98.0%(GC))
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| 英文名称 |
BODIPY 493/503
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| 英文别名 |
Boron,[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-kN)ethyl]-3,5-dimethyl-1H-pyrrolato-kN]difluoro-, (T-4)-;[[(3,5-Dimethyl-1H-pyrrol-2-yl)(3,5-dimethyl-2H-pyrrol-2-ylidene)methyl]methane](difluoroborane);4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene;EverFluor;PYRROMETHENE 546 *FLUORESCENCE REFERENCE STANDARD*;1,3,5,7,8-pentamethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene;4,4-difluoro-1,3,4,5,7-quantmethyl-4-bora-3a,4a-diaza-s-indacene;4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a,diaza-s-indacene;4,4-difluoro-1,3,5,7,8-pentamethyl-BODIPY;Difluoro{2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-N)ethyl]-3,5-dimethyl-1H-pyrrolato-N}boron;Pyrromethene 546;1,3,5,7,8-Pentamethyl-4,4-difluorro-4-bora-3a,4a-diaza-s-indacene;2-[1-(3,5-Dimethyl-2H-pyrrol-2-ylidene)ethyl]-3,,5-dimethyl-1H-pyrrole, boron complex;PM 546;PMPBF2;[[2-[1-(3,5-Dimethyl-2H-pyrrol-2-ylidene)ethyl]-3,5-dimethyl-1H-pyrrolato-N1,N2]difluoro]boron;Pyrromethene 546 (purified by sublimation);1,3,5,7,8-pentaethyl-BODIPY;1,3,5,7,8-Pentamethyl-BODIPY;[[2-[1-(3,5-Dimethyl-2H-pyrrol-2-ylidene)ethyl]-3,5-dimethyl-;503;BODIPY 493;BODIPY(R) 493;D4341;1,3,5,7,8-Pentamethyl-4,4-difluoro-3a-azonia-4a-aza-4-bora(V)-s-indacene-5-ide;{2-[1-(3;BODIPY 493/503
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| Cas No. |
121207-31-6
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| 分子式 |
C14H17BF2N2
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| 分子量 |
262.11
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| 包装储存 |
4°C, sealed storage, away from moistur In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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| 产品详情 |
BODIPY 493/503 (Pyrromethene 546) 是一种荧光中性脂质染料 (Ex/Em: 493/503 nm)。BODIPY 493/503 可用于流式细胞术定量中性脂质含量和显微镜观察脂滴 (LDs)。
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| 生物活性 |
BODIPY 493/503 (Pyrromethene 546) is a fluorescent neutral lipid dye (Ex/Em: 493/503 nm). BODIPY 493/503 can facilitate quantification of neutral lipid content by flow cytometry and observation of lipid droplets (LDs) by microscopy.
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| 性状 |
Solid
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| 体外研究(In Vitro) |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. BODIY staining for flow cytometry1. Grow cells under culture conditions relevant for the study.2. At the time-point of interest, prepare 2 μM BODIPY staining solution in PBS. The volume of staining solution required for each sample corresponds to the volume of media used for culturing cells.3. Wash cells with a quick rinse using 3 mL PBS to remove media/serum.4. Incubate on BODIPY staining solution in the dark for 15 min at 37°C. Include an unstained control for flow cytometry. (Note: From this point, protect samples from light as much as possible)5. Wash cells with a quick rinse using 3 mL PBS to remove staining solution.6. Trypsinize cells to generate a single cell suspension.7.
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
4°C, sealed storage, away from moistur In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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| 参考文献 |
[1]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.
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| 溶解度数据 |
In Vitro: DMSO : ≥ 5 mg/mL (19.08 mM)配制储备液
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1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。
2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。
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