X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system).
性状
Solid
体外研究(In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Dissolve 20 mg X-Gluc Dicyclohexylamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.3. Allow the plates to air dry and be used to inoculate the organisms.4. Incubate the plates at 35°C and observe 16-24 h after inoculation. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
参考文献
[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.