Trypsin (Synonyms: 胰蛋白酶)
目录号: PL10710
CAS No. :9002-07-7
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中文名称
Trypsin
中文别名
胰蛋白酶;胰蛋白酶1:250(猪胰脏);胰蛋白酶1:300(猪胰脏);胰淀粉酶;胰液酵素;胰酶-EDTA溶液;蛋白酶类;胰蛋白酶(猪胰脏);胰蛋白酶(牛胰);胰蛋白酶(牛胰脏);胰蛋白酶 来源于牛胰腺;TPCK-胰蛋白酶;Trypsin 1:250 胰蛋白酶1:250;夫西地酸;牛胰蛋白酶;牛胰腺胰蛋白酶;胰蛋白酶 重组人胰蛋白酶;胰蛋白酶 测序级胰蛋白酶;胰蛋白酶 测序级胰蛋白酶溶液 序列分析纯胰蛋白酶;胰蛋白酶 来源于牛胰腺(0.22 μm,过滤);胰蛋白酶 重组胰蛋白酶;胰蛋白酶(1:300用于组织消化);胰蛋白酶(冷藏);胰蛋白酶(牛胰)1:250;胰蛋白酶(牛胰腺);胰蛋白酶,BR(1:12000);胰蛋白酶,BR(1:125);胰蛋白酶,BR(1:3000);胰蛋白酶,BR(1:3500);胰蛋白酶,TPCK-胰蛋白酶;胰蛋白酶1:25;胰蛋白酶1:250;胰蛋白酶1:2500;胰蛋白酶1:300;胰蛋白酶抑制剂;猪胰腺胰蛋白酶;猪胰腺胰蛋白酶质谱级别;D-色氨酸;精制级,1:2500;胰蛋白酶(猪胰);胰蛋白酶,结晶;胰蛋白酶1:250,Trypsin from bovine pancreas;猪胰脏;胰蛋白酶 来源于牛胰脏
英文名称
Trypsin
英文别名
trypsin;TRYPSIN,1-250;TRYPSIN,1-300;TRYPSIN-EDTA;TRYPSIN,HUMAN PANCREAS;TRYPSIN PORCINE;TRYPSIN, PORCINE PANCREAS;ec3.4.4.4;Trypsin from porcine pancreas;Trypsin from bovin pancreas;TRYPSIN FROM BEEF PANCREAS 2X CRYSTALLIZED;Trypsin from bovine pancreas;Trypsin from bovine pancreas(0.22 βm,Filtered);Trypsin from bovine pancreas(Modified,Sequencing Grade);Trypsin from bovine pancreas(TPCK Treated);Trypsin from bovine pancreas(TPCK-Treated,Irradiated);Trypsin, Human, recombinant, Solution;Trypsin, Porcine, recombinant, Solution;TRYPSIN, CRYSTALLINE;1:250;BAEE >10000 Unit;BR,1:250;D-Tryptophan;potency ≥2500 units;Trypsin Crystallized;Trypsin, DPCC treated, from bovine pancreas;Cocoonase;Parenzyme;Parenzymol;Pseudotrypsin;Trypsin(porcine pancreas);Tryptase;Tryptase from human lung, buffered aqueous solution, >=5 units/mg protein;TPCK Treated
Cas No.
9002-07-7
分子式
C35H47N7O10
分子量
725.79
包装储存
Powder -20°C 3 years;4°C 2 years
产品详情
Trypsin 是一种丝氨酸蛋白酶,可水解赖氨酸或精氨酸羧基侧蛋白质。Trypsin 激活 PAR2 和 PAR4。Trypsin 通过 PDCoV 的 S 糖蛋白与 pAPN 的相互作用诱导 PDCoV 感染的细胞膜融合。Trypsin 还促进细胞增殖和分化。Trypsin 可用于伤口愈合和神经源性炎症的研究。
生物活性
Trypsin is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation.
性状
Solid
IC50 & Target[1][2]
PAR2, PAR4
体外研究(In Vitro)
Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells.
Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells.
Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium.
Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation.
has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究(In Vivo)
Trypsin (100-500?μg per site in 50?μL saline, intradermal injection) induces scratching behaviour in mice. has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Swiss mice
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years;4°C 2 years
ClinicalTrial
参考文献
[1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289.
[2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468.
[3]. H Amano, et al. Trypsin promotes C6 gliom
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2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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