Orexin B, human (TFA) 是 Orexin 受体的内源性激动剂，与OX1和OX2结合的 K_i 值分别为 420 和 36 nM。

Orexin B, human (TFA) is an endogenous agonist at Orexin receptor with K i s of 420 and 36 nM for OX1 and OX2, respectively.

Solid

Ki: 420 nM (OX1), 36 nM (OX2).

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor.
Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC50 of 36 nM in the binding assay and an EC50 of 60 nM in the [Ca]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC50 in the competitive binding assay and the EC50 in the [Ca]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively. has not independently confirmed the accuracy of these methods. They are for reference on

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively. has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model:

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisturePowder -80°C 2 years；-20°C 1 year

RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2

Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:1.  Calculate the length of the peptide.2.  Calculate the overall charge of the entire peptide according to the following table:

[1]. Smart D, et al. Orexins: a new family of neuropeptides. Br J Anaesth. 1999 Nov;83(5):695-7.
[2]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.