Sealed storage, away from moisture and light, under nitrogenPowder -80°C 2 years;-20°C 1 ye
产品详情
Phalloidin-TRITC 是 Phalloidin 的荧光素衍生物,可对肌丝蛋白特异性标记,在标记时可呈现红色荧光,可使用 PE 通道进行荧光成像。
生物活性
Phalloidin-TRITC is a fluorescein derivative of Phalloidin, which can specifically label myof lin and display red fluorescence when labeled and can be observed using Tesred channels.
性状
Solid
体外研究(In Vitro)
1.1 制备储存液用 DMSO 配制 1mM 储存液。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液,终浓度为 200-1000 nM。注:请根据实际情况调整 Phalloidin-TRITC 工作液浓度,且现用现配。2.细胞染色2.1 悬浮细胞:离心收集细胞,加入 PBS 洗涤两次,每次 5 分钟。贴壁细胞:弃去培养基,加入胰蛋白酶消化细胞。离心弃去上清后,加入 PBS 洗涤两次,每次 5 分钟。2.2 加入 1 mL Phalloidin-TRITC 工作液,室温孵育 5-10 分钟。2.3 400 g,4℃ 离心 3-4 分钟,弃去上清。2.4 加入 PBS 洗涤细胞两次,每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后,使用荧光显微镜进行观察。 has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Sealed storage, away from moisture and light, under nitrogenPowder -80°C 2 years;-20°C 1 ye
[1]. J A Cooper, et al. Effects of cytochalasin and phalloidin on actin. J Cell Biol. 1987 Oct;105(4):1473-8.[2]. J A Cooper, et al. Effects of cytochalasin and phalloidin on actin. J Cell Biol. 1987 Oct;105(4):1473-8.[3]. J Wehland, et al. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells