Stains-All，一种阳离子碳菁染料，是研究钙调蛋白 (CaM) 和相关钙结合蛋白 (CaBP) 的各个钙结合位点的结构特征的便捷探针。

Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP).

Solid

Almost all of the proteins found in skeletal muscle extracts, including the Ca+Mg-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca-binding proteins blue, Stains-all is a more sensitive stain for these Ca-binding proteins than is Coomassie blue. has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

[1]. Gruber HE, et al. Application of stains-all for demarcation of cement lines in methacrylate embedded bone. Biotech Histochem. 1991;66(4):181-184.
[2]. Sharma Y, et al. Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin. FEBS Lett. 1993;326(1-3):59-64.

In Vitro: DMSO : 8.33 mg/mL (14.89 mM; ultrasonic and warming and heat to 80°C)配制储备液