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产品总数：60947
| 中文名称 |
Ethidium bromide
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|---|---|
| 中文别名 |
溴化乙啶;溴化-3,8-二氨基-5-乙基-6-苯基菲啶翁;溴化乙锭;溴化乙锭(EB);溴乙菲啶;2,7-二氨基-9-苯基氮杂菲-10-乙基溴;3,8-二氨基-5-乙基-6-苯基菲啶溴化物;菲啶溴红;溴化-3,8-二氨基-5-乙基-6-苯基菲啶;2,7-二氨基-9-苯基氮杂菲-10-乙基溴,菲啶溴红,溴化-3,8-二氨基-5-乙基-6-苯菲啶;溴乙锭(EB);菲啶溴红溶液;菲啶溴红,10MG片剂;菲啶溴红,100MG片剂;溴化乙腚;胡米溴铵;溴乙啶;Ethidium Brmide 溴化乙锭 (EB);Ethidium Bromide 溴化乙啶;溴化乙啶（EB）;溴化乙啶溶液;溴化-3,8-二氨基-5-乙基-6-苯基菲啶翁,溴化乙啶;溴化3,8-二氨基-5-乙基-6-苯基菲啶翁;3,8-二氨基-5-乙基-6-苯基溴化菲啶 (0.5mg/mL于水中);胡米溴铵 (0.5mg/mL于水中);溴化乙锭|EB
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| 英文名称 |
Ethidium bromide
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| 英文别名 |
3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide;2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDINIUM BROMIDE;2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDIUM BROMIDE;3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDINIUM BROMIDE;3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDIUM BROMIDE;3,8-DIAMINO-6-ETHYL-5-PHENYL-PHENANTHRIDIUM BROMIDE;5-ETHYL-3,8-DIAMINO-6-PHENYL-PHENANTHRIDINIUM BROMIDE;CA NUMBERING: 3,8-DIAMINO-5-ETHYL-6-PHENYL-PHENANTHRIDINIUM BROMIDE;ETBR;ETHIDIUM BROMIDE;HOMIDIUM BROMIDE;2,7-Diamino-9-phenyl-10-ethylphenanthridiniumbromide;2,7-Diamino-9-phenylphenanthridineethobromide;3,8-diamino-5-ethyl-6-phenyl-phenanthridiniubromide;Dromilac;Phenanthridinium,3,8-diamino-5-ethyl-6-phenyl-,bromide;Rd1572;Ethidium bromide(EB);Ethidium bromide solution;3,8-diamino-1-ethyl-6-phenylphenantridinium bromide;2.7-Diamino-10-ethyl-9-phenylphenanthridiniumbromide;3,8-Diamino-5;3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide;5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide;3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide (0.5mg/mL in Water);Homidium Bromide (0.5mg/mL in Water);EtBr;Homidium bromide
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| Cas No. |
1239-45-8
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| 分子式 |
C21H20N3+.Br-
|
| 分子量 |
394.31
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| 包装储存 |
4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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| 产品详情 |
Ethidium bromide 常用作分子生物实验学中的荧光标记(核酸染色)，如琼脂糖凝胶电泳。
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|---|---|
| 生物活性 |
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
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| 性状 |
Solid
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| 体外研究(In Vitro) |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).The preparation of agarose gel:1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.2. Mix the agarose powder with 1X TAE/TBE.3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).4. Make the solution cool down before solidification.5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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| 参考文献 |
[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84.[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.
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| 溶解度数据 |
In Vitro: H2O : ≥ 100 mg/mL (253.61 mM)DMSO : 25 mg/mL (63.40 mM; Need ultrasonic)
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1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。
2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。
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