N-Methylmesoporphyrin IX (NMM) 是一种广泛使用的 G-quadruplex DNA 特异性荧光结合剂，是监测 Aβ 纤颤的有效探针。N-Methylmesoporphyrin IX 是一种原位抑制剂，是体外和细胞内 Aβ 淀粉样蛋白生成的非原位监测器。N-Methylmesoporphyrin IX 对 G-四链体 DNA 敏感，但对双链体、三链体和单链形式的 DNA 没有反应。N-Methylmesoporphyrin IX 单独或在单体 Aβ 环境中不发出荧光，但通过与 Aβ 组装体堆叠发出强荧光。

N-Methylmesoporphyrin IX (NMM), a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies.

Solid

By use of N-Methylmesoporphyrin IX (NMM) as an ex situ probe, NMM is added into the incubated Aβ40 solution. The concentration of NMM is fixed at 1μM. To examine the influence of Rhodamine B, 1 μM NMM with 0.1 μM or 0.5 μM Rhodamine B along with 10μM Aβ40 are measured. In the study of using NMM as an in situ inhibitor, NMM (10 μM) is co-incubated with Aβ40 (50 μM) for 7 days at 37 °C. Additional NMM is added before PL measurement to make the concentration of NMM constant (1 μM). The fluorescence spectra of NMM are collected from 550 to 700 nm with an excitation wavelength of 399 nm.
N-Methylmesoporphyrin IX (NMM) is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. Upon binding to quadruplex DNA, for efficient π-π stacking, NMM can adjust its macrocycle geometry to match the terminal face of a G-quadruplex, leading to

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Powder -20°C 3 years；In solvent -80°C 6 months

[1]. Meng Li, et al. N-Methyl Mesoporphyrin IX as an Effective Probe for Monitoring Alzheimers Disease β-Amyloid Aggregation in Living Cells. ACS Chem Neurosci. 2017 Jun 21;8(6):1299-1304.