Piceatannol 3-O-glucoside, an active component of Rhubarb, activates endothelial nitric oxide (NO) synthase through inhibition of arginase activity with IC₅₀s of 11.22 μM and 11.06 μM against arginase I andarginase II, respectively.

Solid

NO synthase
IC50: 11.22 μM (Arginase I), 11.06 μM (Arginase II)

Piceatannol 3-O-glucoside (Piceatannol-3-O-β-D-glucopyranoside; PG) is a potent component of stilbenes, inhibits the activity of arginase I and II prepared from mouse liver and kidney lysates, respectively, in a dose-dependent manner. In human umbilical vein endothelial cells, incubation of Piceatannol 3-O-glucoside markedly blocks arginase activity and increases nitrite and nitrate (NOx) production, as measured by Griess assay. In liver lysates, incubation of different concentrations of Piceatannol 3-O-glucosidesignificantly decreases arginase I activity (75±5% at 1 μM, 72±7% at 3 μM, 62±1% at 10 μM) compared to untreated control (100±9%). In kidney lysates, the residual arginase activities after incubation of 1, 3 and 10 μM Piceatannol 3-O-glucoside are 75±6, 74±5, and 53±8%, respectively. Arginase activity is measured in the presence of different concentration of Piceatan

In order to ascertain whether Piceatannol 3-O-glucoside (PG) ameliorates vascular function in wild-type (WT) and atherogenic model mice [apolipoprotein E-null mice (ApoE)] and to investigate the possible underlying mechanism. Preincubation of aortic vessels from WT mice fed a normal diet (ND) with Piceatannol 3-O-glucoside attenuates vasoconstriction response to U46619 and phenylephrine (PE), while the vasorelaxant response to acetylcholine (Ach) is markedly enhanced in an endothelium-dependent manner. Piceatannol 3-O-glucoside treatment attenuates the phenylephrine (PE)-dependent contractile response, and significantly improves the acetylcholine (Ach)-dependent vasorelaxation response in aortic rings from ApoE mice fed a high-cholesterol diet (HCD). Piceatannol 3-O-glucoside administration in the drinking water significantly reduces fatty streak formation in ApoE mice fed an

Room temperature or refrigerated transport.

4°C, Keep In Dark；*In solvent : -80°C, 6 months; -20°C, 1 month (Keep In Dark)；

Phenols
Polypheno

Plants

体外研究:
DMSO : 50 mg/mL(123.04 mM;Need ultrasonic)
H₂O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)
配制储存液