IQ 1|331001-62-8|-陌孚医药/Medlife

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IQ 1

目录号: PC15898 纯度: ≥98%

CAS No. :331001-62-8

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PC15898-10mM (in 1mL DMSO)	10mM (in 1mL DMSO)	¥776.16	请登录

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中文名称

IQ 1

中文别名

双苯唑菌醇(联苯三唑醇)

英文名称

IQ 1

英文别名

Acetamide, 2-[2-(4-acetylphenyl)diazenyl]-2-(3,4-dihydro-3,3-dimethyl-1(2H)-isoquinolinylidene)-;IQ 1;IQ-1;Acetamide, 2-[2-(4-acetylphenyl)diazenyl]-2-(3,4-dihydro-3,3-dimethyl-1(2H)-isoquinolinylidene...;2-[2-(4-Acetylphenyl)diazenyl]-2-(3,4-dihydro-3,3-dimethyl-1(2H)-isoquinolinylidene)-acetamide

Cas No.

331001-62-8

分子式

C₂₁H₂₄N₄O₂

分子量

364.44

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

生物活性

IQ 1 is a Wnt/β-catenin/CBP signalling sustainer. IQ 1 maintains long-term expansion of Wnt/β-catenin-driven mouse embryonic stem cells (ESCs) and prevents spontaneous differentiation by enhancing β-catenin/CBP-mediated transcription and preventing conversion to β-catenin/p300-mediated transcription. IQ-1 regulates Wnt signalling by interacting with PR72/130. IQ 1 can be used in study of ESCs expansion.

性状

Solid

体外研究(In Vitro)

IQ-1 (1.10, 3.48, 11.04 mM; 7 days) maintains the undifferentiated state of ESCs.
IQ-1 (0.28, 1.10, 2.76, 11.04 mM; 21 h) maintains murine ESC self-renewal independently of LIF.
IQ-1 Binds the PR72/130 Subunit of the Serine/Threonine Phosphatase PP2A in P19 cells.
IQ-1 (10 μM; 24 h) modulates Wnt signaling via interaction with PR72/130 in P19 cells.
IQ-1 (10 μM; 24 h) indirectly decreases the phosphorylation of p300 Ser-89 and Thereby the β-Catenin/p300 interaction.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Embryonic stem cells (ESCs)
Concentration:	1.10, 3.48, 11.04 mM
Incubation Time:	7 days
Result:	Dose dependently increased alkaline phosphatase activity, in media containing 15% FCS without the addition of exogenous leukemia inhibitory factor (LIF). Maintained SSEA-1 (undifferentiated ESC marker) expression dose-dependently.

RT-PCR

Cell Line:	Embryonic stem cells (ESCs)
Concentration:	0.28, 1.10, 2.76, 11.04 mM
Incubation Time:	21 h
Result:	Significantly increased and maintained Nanog expression in culture. (Nanog: a divergent homeoprotein pluripotency sustaining factor for ESCs that drives ESC self-renewal). Exhibited the maintenance of murine ESC pluripotency were independent of the LIF/Stat3 pathway.

Western Blot Analysis

Cell Line:	P19 cells
Concentration:	10 μM
Incubation Time:	24 h
Result:	Significantly reduced coimmunoprecipitation of PR72/130 with both PP2A and Nkd. Binded to PR72/130 subunit of PP2A, and disrupted the PP2A/Nkd complex. Decreased the affinity of the β-catenin/p300 interaction and increases β-catenin/CBP usage by negatively regulating the phosphorylation of p300 Ser-89.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

[1]. Miyabayashi T, et al. Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5668-73. [Information]

溶解度数据

体外研究:

DMSO : ≥ 33 mg/mL (91.05 mM)

* "≥" means soluble, but saturation unknown.

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.7592 mL	13.7961 mL	27.5923 mL
5 mM	0.5518 mL	2.7592 mL	5.5185 mL
10 mM	0.2759 mL	1.3796 mL	2.7592 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution

此方案可获得 ≥ 1.67 mg/mL (4.61 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液

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产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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