2,3-Butanedione-2-monoxime|57-71-6|(Diacetyl monoxime; DAM)-陌孚医药/Medlife

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2,3-Butanedione-2-monoxime (Synonyms: Diacetyl monoxime; DAM)

目录号: PC12413 纯度: ≥98%

CAS No. :57-71-6

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PC12413-100g	100g	¥637.00	请登录
PC12413-10mM (in 1mL DMSO)	10mM (in 1mL DMSO)	¥490.00	请登录

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中文名称	2,3-Butanedione-2-monoxime
中文别名	联乙酰一肟;2,3-丁二酮一肟;挡料圈,空气阀,冷却水包;丁二酮单肟;二乙酰一肟;甲基肟乙基甲酮;2,3-丁二酮肟;二甲基乙二酮单肟;2,3-丁烷二酮一肟;Diacetyl Monoxime 二乙酰一肟;丁酮肟;二乙酰单肟;二乙酰肟;二乙酰一肟,AR;2,3-丁烷二酮-2-肟;丁二酮一肟;二甲基乙二酰一肟;二乙酰一肟,Diacetylmonoxime ,AR;双乙酰一肟;异亚硝基甲基乙基甲酮;2,3-丁二酮-2-肟;异亚硝基乙基甲基甲酮;肟乙基甲酮
英文名称	2,3-Butanedione-2-monoxime
英文别名	2,3-Butanedione monoxime;2,3-butanedione oxime;Diacetyl monoxime;ISONITROSOETHYL METHYL KETONE;DAM;DIACETYL MONOOXIME;BDM;BIACETYL MONOOXIME;BIACETYL MONOXIME;2,3-BUTANEDIONE, MONO-OXIME;2.3-Butanedionemonoxime;DIACETYL MONO-OXIME,CP;Isonitroethyl Methyl ketone;IsonitroMethyl ethyl ketone;Methyl isonitroethyl ketone;2,3-Butanedione 2-Oxime;butanedione monoxime;3-hydroxyiminobutan-2-one;FSEUPUDHEBLWJY-UHFFFAOYSA-N;Butane-2,3-dione monooxime;3-(hydroxyimino)butan-2-one;2,3-BUTANEDIONE-2-OXIME;butanedione mono-oxime;2,3-butanedione mono-oxime;butane-2,3-dione-2-oxime;KBioSS_002489;KBioGR_002482;KBio2_002482;KBio3_002960;KBio2_007618;KBio2_005050;Bio1_000469;Bio1_001447;Bio1_000958;(e)-3-(hydroxyimino)butan-2-one;AK401871;TL8003706;FT-
Cas No.	57-71-6
分子式	C₄H₇NO₂
分子量	101.10
包装储存	4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

生物活性

Biacetyl monoxime (Diacetyl monoxime), a myosin ATPase inhibitor, is a skeletal and cardiac muscle contraction inhibitor. Biacetyl monoxime is also a well-characterized non-competitive inhibitor of chemical and motile activity of skeletal muscle myosin-II. Biacetyl monoxime induces sarcoplasmic reticulum Ca release.

性状

Solid

体外研究(In Vitro)

Biacetyl monoxime (Diacetyl monoxime) (50 mM, 6 and 48 h) decreases cellulase secretion in C. cinerea.
Biacetyl monoxime (50 mM, 2 and 4 h) disrupts the localization of the Golgi apparatus, but not that of the endoplasmic reticulum.
Biacetyl monoxime (0-30 mM) induces SR Ca release (no efflux inhibitors) in a concentration-dependent manner, with a maximal reduction of 72% of SR Ca at pCa 6.0.
Biacetyl monoxime acts as a chemical phosphatase, which has led to speculation that dephosphorylation of key Ca channel proteins may be involved in its inhibition of contraction.
Biacetyl monoxime does not inhibit the ATPase activity of two different myosin-I isoforms, myosin-V, or myosin-VI.
Biacetyl monoxime (0-50 mM) suppresses L-type Ca current of single cardiac myocytes isolated from SHR and WKY rats.
Biacetyl monoxime significantly reduces the duration of both spontaneous and electrically stimulated action potentials of cultured neonatal rat cardiomyocytes.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究(In Vivo)

Biacetyl monoxime (0-200 mg/kg; i.v.; once) shows hypotensive effect.
Biacetyl monoxime (0-205 mg/kg; i.p.; once) shows anticonvulsant effect against Picrotoxin (HY-101391)-induced convulsions.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male SHR and age-matched WKY rat
Dosage:	5, 30, I00 and 200 mg/kg
Administration:	Intravenous administration, 1 mL/kg, once
Result:	Decreased arterial blood pressure for both strains, the SHR was significantly more responsive.

Animal Model:	Male mice (20 to 25 g)
Dosage:	51, 103 and 205 mg/kg in combination with intraperitoneal injection of 3.0 mg/kg Picrotoxin (HY-101391)
Administration:	Intraperitoneal injection, once
Result:	Showed dose-dependent anticonvulsant effect against Picrotoxin-induced convulsions.

运输条件

Room temperature or refrigerated transportation.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献

[1]. Kohsuke Hashimoto, et al. The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea. Biotechnol Lett. 2011 Apr;33(4):769-75. [Information]

[2]. R M Phillips, et al. 2,3-Butanedione 2-monoxime (BDM) induces calcium release from canine cardiac sarcoplasmic reticulum. Biochem Biophys Res Commun. 1996 Dec 4;229(1):154-7. [Information]

[3]. Ostap EM. 2,3-Butanedione monoxime (BDM) as a myosin inhibitor. J Muscle Res Cell Motil. 2002;23(4):305-8. [Information]

[4]. Xiao YF, et al. Effects of 2,3-butanedione monoxime on blood pressure, myocardial Ca2+ currents, and action potentials of rats. Am J Hypertens. 1995 Dec;8(12 Pt 1):1232-40. [Information]

[5]. Brightman T, et al. 2,3-Butanedione monoxime protects mice against the convulsant effect of picrotoxin by facilitating GABA-activated currents. Brain Res. 1995 Apr 24;678(1-2):110-6. [Information]

溶解度数据

体外研究:

DMSO : 125 mg/mL (1236.40 mM; Need ultrasonic)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	9.8912 mL	49.4560 mL	98.9120 mL
5 mM	1.9782 mL	9.8912 mL	19.7824 mL
10 mM	0.9891 mL	4.9456 mL	9.8912 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (20.57 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液
2.

建议依照次序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (20.57 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

建议依照次序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (20.57 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (20.57 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

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产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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