Title：In vascular smooth muscle cells paricalcitol prevents phosphate-induced Wnt/beta-catenin activation

标题：在血管平滑肌细胞中，帕里卡托尔能防止磷酸盐诱导的Wnt/β-连环蛋白激活

Authors: Martinez-Moreno JM, Munoz-Castaneda JR, Herencia C, et al.

Journal: American Journal of Physiology - Renal Physiology, 2012

DOI: 10.1152/ajprenal.00262.2012

Abstract

This study investigates the effects of paricalcitol, a vitamin D analog, on vascular smooth muscle cells (VSMCs) in the context of phosphate-induced activation of the Wnt/β-catenin signaling pathway, which is a critical factor in vascular calcification. The researchers found that paricalcitol can inhibit phosphate-induced activation of this pathway, thus potentially preventing vascular calcification.

摘要

本研究旨在探讨维生素D类似物帕立骨化醇（paricalcitol）对血管平滑肌细胞（VSMCs）中磷酸盐诱导的Wnt/β-连环蛋白信号通路激活的抑制作用。研究发现，磷酸盐会诱导Wnt/β-连环蛋白信号通路的激活，导致VSMCs的钙化。帕立骨化醇能够有效地阻止这种激活，从而可能预防血管钙化。

Key Findings

1. **Phosphate-Induced Activation**: Phosphate was shown to induce the Wnt/β-catenin pathway in VSMCs, leading to vascular calcification.

2. **Role of Paricalcitol**: Paricalcitol effectively prevented the activation of the Wnt/β-catenin pathway in these cells.

3. **Mechanism of Action**: The study suggests that paricalcitol's inhibition of this pathway is a potential mechanism for its protective effects against vascular calcification.

主要发现

1. **磷酸盐诱导的激活**: 磷酸盐在血管平滑肌细胞中诱导了Wnt/β-连环蛋白信号通路的激活，这与血管钙化相关。

2. **帕立骨化醇的作用**: 帕立骨化醇有效地抑制了磷酸盐诱导的Wnt/β-连环蛋白信号通路的激活。

3. **作用机制**: 帕立骨化醇通过抑制Wnt/β-连环蛋白信号通路，展示了其预防血管钙化的潜力。

Antifungal Compounds and CAS Numbers

1. **Paricalcitol (帕立骨化醇)**
   - CAS Number: 131918-61-1

2. **Phosphate (磷酸盐)**
   - Various forms such as disodium phosphate (CAS Number: 14265-44-2)

Study Content

**Research Background**: High phosphate levels are common in patients with chronic kidney disease and are closely related to vascular calcification.

**Research Objective**: To explore the role of paricalcitol in preventing phosphate-induced calcification of VSMCs.

**Methods**: Experiments were conducted using cultured VSMCs, and the activation of the Wnt/β-catenin signaling pathway was assessed to evaluate the effect of paricalcitol.

**Results**: Paricalcitol significantly inhibited the phosphate-induced activation of the Wnt/β-catenin signaling pathway.

研究内容

**研究背景**: 高磷酸盐水平在慢性肾病患者中常见，并与血管钙化密切相关。

**研究目标**: 探讨帕立骨化醇在防止磷酸盐诱导的VSMCs钙化中的作用。

**方法**: 使用体外培养的VSMCs进行实验，通过检测Wnt/β-连环蛋白信号通路的激活情况来评估帕立骨化醇的效果。

**结果**: 帕立骨化醇显著抑制了磷酸盐诱导的Wnt/β-连环蛋白信号通路的激活。