Authors: Billen B, Brams M, Debaveye S, Remeeva A, Alpizar YA, Waelkens E, et al.

Journal: Journal of Biological Chemistry

Publication Date: May 15, 2015

Volume: 290

Issue: 20

Pages: 12964-12974

DOI: 10.1074/jbc.M114.628925

Abstract:

This study investigates the TRPV3 cation channel, focusing on how different ligands induce unique conformational changes. Using intrinsic tryptophan fluorescence quenching, the researchers analyzed the structural dynamics of the TRPV3 channel. They discovered that specific ligands interact differently with the channel, leading to distinct conformational states. This work enhances the understanding of ligand-specific effects on TRPV3 channel functionality, which is significant for physiological processes and potential therapeutic applications.

摘要：

本研究探讨了TRPV3阳离子通道，重点研究了不同配体如何诱导独特的构象变化。通过内在色氨酸荧光猝灭，研究人员分析了TRPV3通道的结构动态。他们发现，特定配体与通道的相互作用不同，导致不同的构象状态。这项工作增强了对配体特异性对TRPV3通道功能影响的理解，这对于生理过程和潜在的治疗应用具有重要意义。

Key Findings:

1. Ligand Interaction: Different ligands cause unique conformational changes in the TRPV3 channel.
2. Fluorescence Quenching: Intrinsic tryptophan fluorescence quenching was utilized to observe these changes.
3. Structural Dynamics: The study provides insights into the structural dynamics of the TRPV3 channel.

主要发现：

1. 配体相互作用： 不同配体导致TRPV3通道独特的构象变化。
2. 荧光猝灭： 使用内在色氨酸荧光猝灭观察这些变化。
3. 结构动态： 该研究提供了TRPV3通道结构动态的见解。

Methodology:

• Intrinsic Tryptophan Fluorescence Quenching: Used to measure conformational changes.
• Ligands Studied: Various ligands were tested to determine their specific effects on the TRPV3 channel.

方法学：

• 内在色氨酸荧光猝灭： 用于测量构象变化。
• 研究的配体： 测试了各种配体以确定其对TRPV3通道的具体影响。

相关CAS号：

·  Eugenol (CAS: 97-53-0): A natural compound often used in dental practices for its analgesic properties, known to activate TRPV3.

·  Carvacrol (CAS: 499-75-2): A monoterpenoid phenol found in oregano oil, also acts as a TRPV3 activator.

·  2-Aminoethoxydiphenyl borate (2-APB) (CAS: 524-95-8): A chemical that modulates TRP channels, including TRPV3.

·  Camphor (CAS: 76-22-2): A terpene commonly used in medicinal products, which can activate TRPV3.