Title：Collection and Identification of Allelopathic Compounds from the Undisturbed Root System of Bigalta Limpograss (Hemarthria altissima)

标题：从大黎草（Hemarthria altissima）未受干扰的根系中分离鉴定化感物质

Authors: Tang CS, Young CC
作者: 唐CS, 杨CC
Journal: Plant Physiology
期刊: 植物生理学
Publication Date: January 1982
出版日期: 1982年1月
Volume: 69
卷号: 69
Issue: 1
期号: 1
Pages: 155-160
页码: 155-160

Abstract / 摘要

This study introduced a method to continuously collect allelopathic compounds from the intact root system of Bigalta limpograss using a nutrient solution circulated through a column containing XAD-4 resin. The method enabled the identification of 12 primary phenolic compounds with growth-regulating activities.
本研究提出了一种新的方法，通过将营养溶液循环通过含有XAD-4树脂的柱子，连续收集未受干扰的Bigalta草丛根系中的化感化合物。该方法能够有效地收集植物根系分泌的代谢产物，并避免土壤中的高污染水平干扰。研究识别了12种主要的酚类化合物，这些化合物具有已知的生长调节活性。

Methods / 方法

1. Culture Method: Using a sand culture system with Bigalta limpograss.
1. 培养方法: 使用含有Bigalta草丛的沙培系统。
2. Collection System: Nutrient solution circulated through the root system and XAD-4 resin column to adsorb hydrophobic metabolites from the root exudates.
2. 收集系统: 营养溶液循环通过根系和XAD-4树脂柱，吸附根系分泌的疏水代谢物。
3. Separation and Analysis: Eluted the resin with methanol and separated the compounds using paper and thin-layer chromatography. Identified the compounds using gas chromatography-mass spectrometry (GC-MS).
3. 分离和分析: 使用甲醇洗脱树脂，并通过纸层析和薄层层析分离化合物。使用气相色谱-质谱联用技术（GC-MS）进行鉴定。

Results / 结果

1. Identified 12 phenolic compounds, including 3-hydroxycinnamic acid, benzoic acid, phenylacetic acid, and cinnamic acid.
1. 鉴定出12种酚类化合物，其中包括3-羟基肉桂酸、苯甲酸、苯乙酸和肉桂酸。
2. The method is more efficient compared to the traditional solvent extraction method and is suitable for a wide range of studies on rhizosphere chemistry.
2. 方法相比传统溶剂提取法更为高效，适用于根际化学方面的广泛研究。

Phenolic Compounds with CAS Numbers / 酚类化合物及其CAS号

1. 3-Hydroxycinnamic acid (Caffeic acid): CAS No. 331-39-5
1. 3-羟基肉桂酸（咖啡酸）: CAS号 331-39-5
2. Benzoic acid: CAS No. 65-85-0
2. 苯甲酸: CAS号 65-85-0
3. Cinnamic acid: CAS No. 140-10-3
3. 肉桂酸: CAS号 140-10-3