Title：Effects of amiodarone and posaconazole on the growth and ultrastructure of Trypanosoma cruzi

标题：盐酸胺碘酮和泊沙康唑对克鲁兹锥虫生长及超微结构的影响

Authors: Veiga-Santos P, Barrias ES, Santos JF, de Souza W, Soeiro MN

作者: Veiga-Santos P, Barrias ES, Santos JF, de Souza W, Soeiro MN

Journal: International Journal of Antimicrobial Agents

期刊: 国际抗微生物剂杂志

Publication Date: July 2012

发表日期: 2012年7月

Volume and Issue: Volume 40, Issue 1

卷和期: 第40卷，第1期

Pages: 61-71

页数: 61-71

Abstract: The study investigates the effects of two drugs, amiodarone and posaconazole, on the protozoan parasite Trypanosoma cruzi, which is responsible for Chagas disease. The researchers analyzed both the growth inhibition and the ultrastructural changes induced by these drugs in the parasite.

摘要: 本研究调查了两种药物，胺碘酮和泊沙康唑，对引起恰加斯病的原生动物寄生虫锥虫（Trypanosoma cruzi）的影响。研究人员分析了这些药物对寄生虫生长抑制和超微结构变化的影响。

Key Findings:

1. Growth Inhibition: Both amiodarone and posaconazole demonstrated significant inhibitory effects on the growth of T. cruzi. The combination of these drugs showed enhanced efficacy.
2. Ultrastructural Changes: Treatment with amiodarone and posaconazole led to notable ultrastructural alterations in the parasite, including changes in the plasma membrane, mitochondria, and other organelles.
3. Mechanism of Action: The study suggests that these drugs disrupt sterol biosynthesis and calcium homeostasis in T. cruzi, leading to impaired parasite viability.

主要发现:

1. 生长抑制: 胺碘酮和泊沙康唑均表现出显著的抑制锥虫生长的效果。两种药物的组合显示出增强的疗效。
2. 超微结构变化: 胺碘酮和泊沙康唑的治疗导致寄生虫的超微结构发生显著变化，包括质膜、线粒体和其他细胞器的变化。
3. 作用机制: 研究表明，这些药物通过破坏锥虫的甾醇生物合成和钙稳态，从而影响寄生虫的生存能力。

Methodology:

• The growth of T. cruzi was monitored in the presence of varying concentrations of amiodarone and posaconazole.
• Electron microscopy was used to observe the ultrastructural changes in the parasites after drug treatment.
• Biochemical assays were conducted to analyze the disruption of sterol biosynthesis and calcium homeostasis.

方法学:

• 在不同浓度的胺碘酮和泊沙康唑存在下，监测锥虫的生长情况。
• 使用电子显微镜观察药物治疗后寄生虫的超微结构变化。
• 进行生化分析以分析甾醇生物合成和钙稳态的破坏。

Conclusion: The findings highlight the potential of amiodarone and posaconazole as effective treatments for Chagas disease, particularly when used in combination. The study provides a foundation for further research into the therapeutic applications of these drugs.

结论: 研究结果突显了胺碘酮和泊沙康唑作为治疗恰加斯病的有效药物的潜力，尤其是在联合使用时。该研究为这些药物的进一步治疗应用研究奠定了基础。

Compounds and CAS Numbers

1. Amiodarone (胺碘酮)

   - CAS Number: 1951-25-3

2. Posaconazole (泊沙康唑)

   - CAS Number: 171228-49-2